Effect of Extract of Carapax et Plastrum Testudinis on Nuclear Receptor in the Proliferation of Rat Mesenchymal Stem Cell in Vitro
- VernacularTitle:龟甲提取物对骨髓间充质干细胞增殖过程中核受体的影响
- Author:
Shucai SONG
;
Hua XU
;
Jianhong ZHOU
;
Hui LI
;
Yiwei LI
;
Shaohui DU
;
Dongfeng CHEN
- Publication Type:Journal Article
- Keywords:
EXTRACT OF CARAPAX ET PLASTRUM TESTUDINIS/phaimacology;
MESENCHYMAL STEM CELLS/drug effects;
CELL PROLIFERATION;
NUCLEAR RECEPTORS/pathology;
CELL CULTURE;
RATS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To observe the effect of extract of Carapax et Plastrum Testudinis (ECPT) on nuclear receptor in the proliferation of rat mesenchymal stem cell (MSC) in vitro. [Methods] The rat MSC dissociated from bone marrow by density gradient method were cultured and identified by marking of bromodeoxyuridine (Brdu) and staining of CD44. Then different doses of ECPT (3333, 333.3 and 33.33 ?g/mL) were respectively added into in-vitro cultured MSC for 12, 24, 72 and 120 hours. The expression of retinoic acid receptor-?(RAR?), vitamin D receptor (VDR), estrogen receptor (ER), glucocorticoid receptor (GR), thyroid hormone receptor-?(TR?) and peroxisome proliferator-activated receptor ?(PPAR?) in MSC was detected by immunohistochemistry and immunofluorescence methods. [Results] The number of RAR?-and VDR-positive cells in ECPT groups was higher than that in the control group (P
