Level of urocortin mRNA during labor and eff ect of urocortin on myometrial contractility in vitro
- VernacularTitle:胎盘和子宫平滑肌组织中尿皮质素mRNA表达水平变化及其与分娩发动的关系
- Author:
Tao SHANG
;
Zhaoxia CHENG
;
Feng JIN
;
Li ZHANG
- Publication Type:Journal Article
- Keywords:
Corticotropin-releasing hormone;
Myometri um;
Placenta;
Uterine contraction
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of urocortin mRNA during labor and the effect of uroco rtin on myometrial contractility, and to investigate its role in the onset and p rogress of labor. Methods (1) Semi-quantitative reverse transcription-polymerase chain reaction (RT -PCR),using ?-actin as internal standard was applied to determine the levels of urocortin mRNA in human placenta and myometrium from the group of cesarean se ction before (10 cases) and during (10 cases in latent phase and 10 cases in act ive phase) labor.(2) The isolated myometrial strips of pregnant women (n=24 ) were prepared.The effects of urocortin with or without prostaglandin F 2? (PGF 2?) and oxytocin on myometrial contractility were evaluated by area s under the curve. Results (1) Semi-quantitative RT-PCR showed that the expression level of urocortin mRN A in placenta and myometrium after the onset of labor were higher than before la bor (1.23 ?0.52, 1.32?0.22; 0.83?0.38, 0.94?0.13, respectively, P0.05). Conclusion The study indicates urocortin may indirectly m odulate myometrial contractility during labor.
