Extraction of total-saponins from Patrinia Villosa Juss based on uniform design method
- VernacularTitle:均匀设计法优选白花败酱总皂苷的提取工艺
- Author:
Jiexin XU
;
Fangqin ZHOU
;
Fangming JIANG
;
Yanzhi LIAO
- Publication Type:Journal Article
- Keywords:
uniform design method;
Patrinia Villosa Juss;
total-saponins content;
extraction technique
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To confirm the optimum extraction of the total-saponins from Patrinia Villosa Juss. METHODS: Optimum conditions(ethanol concentration,extraction hours,ethanol solution quantity) were determined by the uniform design test,the extract was refined with macropore resin AB-8,and samples were measured colorimetrically at ?=560 nm compared with oleanolic acid as reference substance. RESULTS: The result was 95% ethanol as solvent, extraction time for 1 h adding 8 times amount of ethanol solution at 100 ℃ water bath for two times.under this condition,the total-saponins content was 0.668%,in accordance with the design forecast. CONCLUSION: The optimum extraction technique and purified methods could extract the total-saponins efficiently from Patrinia Villosa Juss.The technique is simple and adapts for production.
