Gentiopicroside content in violet Gentian by LC-MS and HPLC
- VernacularTitle:藏药紫花龙胆中龙胆苦苷的LC-M S定性和HPLC定量分析
- Author:
Xiangyan LIANG
;
Qiong TIAN
;
Chaofeng LI
- Publication Type:Journal Article
- Keywords:
LC-MS;
MS/MS;
violent gentiana;
gentiopicroside
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To identify and quantitative analysis gentiopicroside content in the extract of Violent gentian of Tibetan herbal medicine. METHODS: Application of HPLC-the electricity sprays fog-mass spectroscopy/mass spectroscopy(HPLC-ESI-MS/MS) and the technique of LC-DAD-UV,by contrast with the reservation time of the authentic gentiopicroside,the ultraviolet absorbed spectrum,the molecule~+,quickly identified the gentiopicroside in the extract of violent gentian.As to it's quantitative analyis we adopted the capillary HPLC methods,with the weight of authentic gentiopicroside for abscissa. RESULTS: MS showed that the methanol extract of violet gentian mainly had five peaks.the compound structure of first peak was gentiopicroside,molecule~+ 356.9,result showed that gentiopicroside in gentian was 1.97%. CONCLUSION: Gentiopicroside is the mainly component of violet Gentia.
