Recombinant human bone morphogenetic protein-2 stimulation of cartilage regeneration in canine tracheal graft
- VernacularTitle:RhBMP-2诱导犬气管移植体软骨再生的实验研究
- Author:
Xiaofei LI
;
Jian WANG
;
Tao ZHANG
- Publication Type:Journal Article
- Keywords:
Trachea Bioprosthesis Cartilage Regeneration
- From:
Chinese Journal of Thoracic and Cardiovascular Surgery
2003;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To induce regeneration of dog's tracheal cartilage by implanting the recombinant human bone morphogenetic protein-2 (rhBMP-2) into the tracheal graft in order to prevent the trachea from collapse. Methods RhBMP-2 with the atelopeptide type-1 collagen carrier was implanted around the cartilage ring of the autograft. New cartilage formed was detected with the histological analysis and immunohistochemical stain after 4 weeks. Six different concentrations of rhBMP-2 with collagen carrier were implanted around the cartilage ring in 6 auto transplantation groups respectively. The area of new cartilage regeneration was calculated in choosing the optimal dosage of rhBMP-2. Auto and allo tracheal transplantation were performed with rhBMP-2 implanted around the cartilage ring, the diameter of the grafts, histological analysis, and the area of the new cartilage regeneration were calculated. Results The addition of rhBMP-2 resulted in significantly greater amount of the new cartilage area (P
