Fingerprints of Qingkailing Injection by HPLC/UV/MS/MS
- VernacularTitle:清开灵注射液HPLC/UV/MS/MS指纹图谱研究
- Author:
Hongyang ZHANG
- Publication Type:Journal Article
- Keywords:
Qingkailing Injection;
fingerprints;
HPLC/UV/MS/MS;
qualitative identification
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To develop a method of the HPLC/UV/MS/MS fingerprints of Qingkailing Injection(Radix Isatidis,Fructus Gardeniae,Flos Lonicerae Japonicae,etc.) and provide a novel conception for its quality control. METHODS: Separation was performed on Phenomenex Luna C_(18) analytical column(250 mm?4.6 mm,5 ?m).The mobile phases consisted of water containing 0.1% formic acid and acetonitrile was used as gradient elute.The flow rate was 1 mL/min.An Electrospray Ion Trap mass spectrometer was utilized for qualitative analysis and both positive and negative secondary ion scan mode were applied. RESULTS: Perfect fingerprints with excellent separation and reproducibility were obtained and 13 compounds were identified.Especially three isomers,including Neochlorogenic acid,Chlorogenic acid and Isochlorogenic acid,were differentiated successfully. CONCLUSION: The method is suitable for establishing the HPLC/MS/MS fingerprints of Qingkailing Injection and hence could be used as an effective and credible pattern for analyzing traditional Chinese medicine as well as its quality control.Moreover,based on this approch,further traditional Chinese medicine research can be performed.
