Analysis of flavonoids in seeds of Oroxylum indicum by HPLC-ESI-MS
- VernacularTitle:木蝴蝶中黄酮类化合物的HPLC-ESI-MS分析
- Author:
Jianrong MIAO
- Publication Type:Journal Article
- Keywords:
Oroxylum indicum(L.) Vent.;
HPLC-ESI-MS;
flavone
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC-ESI-MS method for simultaneous analysis and determination of flavone in the seeds of Oroxylum indicum.Methods The separation was performed by Hypersil C_(18) column(250 mm?4.6 mm,5 ?m) with a mobile phase of formic acid aqueous solution(A) and acetonitrile(B) 0.5%.The flow rate was 1.0 mL/min and detection wavelength was at 195-400 nm.The samples were analyzed in positive mode.Results Six flavones in the seeds of O.indicum could be separated in one run.Baicalin,chrysin-7-glucuronide, oroxylin A,and baicalin-7-O-glucoside had been tentatively (identified) by ESI-MS and reference data.Conclusion Besides quantification of the components,this new method surpasses previously published ones in product quality control with accurate and rapid advantage,and provides the HPLC chromatographic fingerprints of biological active components in the seeds of O.indicum.
