Quantitative determination of triptoquinone B in Radix Folium Seu Flos Tripterygii Wilfordii and its tablets by HPLC
- VernacularTitle:HPLC法测定雷公藤及其片剂中雷公藤对醌B
- Author:
Jiangping HE
;
Wenyan WANG
;
Wenyuan GAO
;
Yanwen ZHANG
;
Qingwei ZHANG
;
Wen ZHOU
;
Hongquan DUAN
- Publication Type:Journal Article
- Keywords:
Radix Folium Seu Flos Tripterygii Wilfordii(RFFTW);
Tripterygium wilfordii preparations;
triptoquinone B;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for determination of triptoquinone B in Radix Folium Seu Flos Tripterygii Wilfordii(RFFTW) and its tablets.Methods An external method with HiQ siL KYA-C_(18)(250 mm?4.6 mm,5 ?m) column as fixed phase and methanol-1% acetic acid solution((66∶)34) as mobile phase was adopted.The detective wave length was 254 nm and the flow rate was 1.0 mL/min.Results The linearity range was 6.62—105.9 ?g/mL(r=0.999 9),the average recovery of T.wilfordii from Hunan Province was 97.78%,RSD was 1.09%(n=9),and the average recovery of tablet of T.hypoglaucum was 99.83%,RSD was 1.76%(n=9).Conclusion The method is accurate and sensitive.It is adoptable for quantitative analysis of triptoquinone B in RFFTW and its tablets.
