IEF Electrophorogram of protein in Rhizoma Pinelliae
- VernacularTitle:半夏药材中蛋白质IEF电泳图谱的研究
- Author:
Bisheng HUANG
;
Keli CHEN
;
Yan CAO
- Publication Type:Journal Article
- Keywords:
Pinellia ternata(Thunb.) Breit.;
P.pedatisecta Schott;
Typhonium flagelliforme(Lodd.) Blume;
protein electroforesis,isoelectrofocus(IEF)
- From:
Chinese Traditional and Herbal Drugs
1994;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discriminate Rhizoma Pinelliae from its relative species by electrophoro-gram of protein.Methods Using isoelectrofocusing(IEF) technology to compare the electrophorogram of tuber of Pinellia ternata produced in different habitats and from its relative plants then determining the pH values.Results The IEF electrophorogram of Rhizoma Pinelliae was stable with eight clear characteristic protein bands and had obvious differences from the electrophorogram of the tubers of its relative plants.Conclusion The IEF electrophoresis of Rhizoma Pinelliae can be used as the distinguishing basis.The PI value of the characteristic protein of P.ternana offers the basic parameter for separation and purification of the protein in Rhizoma Pinelliae.
