Determination of Adenosine in Chongcaoqizhi Granule by HPLC
- VernacularTitle:高效液相色谱法测定虫草杞芝颗粒中腺苷的含量
- Author:
Mei QI
;
Hui LIU
- Publication Type:Journal Article
- Keywords:
Chongcaoqizhi granule;
adenosine;
RP-HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a RP-HPLC method to determine adenosine in Chongcaoqizhi granule. Method The separation was performed in a Kromasil ODS-1 column (250 mm?4.6 mm, 5 ?m) with a mobile phase of phosphate buffer solution (pH 6.5) - methanol (85∶15). The flow was 0.7 mL/min and the detective wavelength was set at 260 nm. Results The standard adenosine showed a good linear correlation at range of 1.016~12.192 ?g/mL. The linear regression equation and correlation coefficient (r) were Y =169339X -82318, r =0.9990, respectively. The average recovery of the loading was 98.02% and the RSD was 1.72%. The average content of adenosine was 12.45 ?g/g. Conclusion This method is accurate, reproducible and highly selective, and can be used for quality control of Chongcaoqizhi granule.
