The significance of NSE and S100? protein in the diagnosis of early brain injury after cardiopulmonary resuscitation in rats
- VernacularTitle:心肺复苏后大鼠血清神经元特异性烯醇化酶、S100?蛋白对脑损伤诊断的价值
- Author:
Yujie MA
;
Xingyi YANG
;
Zhaofen LIN
;
Lei ZHANG
- Publication Type:Journal Article
- Keywords:
Cardiopulmonary resuscitation;
Brain damage;
Phosphopyruvate hydratase;
Nerve tissue protein S100?
- From:
Chinese Journal of Emergency Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the changes of neuron specific enolase(NSE) and S100? protein levels in serum after cardiopulmonary resuscitation(CPR) in rats,and and to evaluate their significance on early diagnosis of brain injury. Methods Caridiac arrest model was induced by asphyxiation with ice-cold 0.5 mol KCl in rats,and resuscitation 5 minutes after arrest.Fifty male Sprague Dawley rats were randomly divided into 5 groups : sham control group (n=10) and 4 routine treatment group (experimental group) (n=10,per group) . The blood samples were taken from rats at 6 , 12 , 24 , 48 hours after restoration of spontaneous circulation(ROSC).NSE and S100? measured by enzyme-linked immunosorbent assay were compared between each experimental group,serum from sham control animals was also analyzed. Results Both NSE and S100? were significantly increased in serum after ROSC when compared to sham controls(P
