THE UP-REGULATED EXPRESSION OF HEAT-SHOCK-PROTEIN 27(Hsp27) IN INJURED RETINA GANGLION CELLS WITH METHYLPREDNISOLONE TREATMENT
- VernacularTitle:甲基强的松龙上调受损视网膜节细胞内Hsp27蛋白的表达
- Author:
Qunyu PEI
;
Lei YANG
;
Ke WANG
;
Liyuan YANG
;
Changman ZHOU
;
Jiliang LEI
- Publication Type:Journal Article
- Keywords:
Retina ganglion cells(RGCs);
Methylprednisolone(MP);
Early protection;
Heat-Shock-Protein 27(Hsp27);
Immunohistochemical staining;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study methylprednisolone's effect of up-regulation of Hsp27 on provocation and enhancement of self-protection and self-repair to injured central nerves,and if the injured nerves can get protection in early period,and therefore benefit injured central nerves'survival and regeneration. Methods Optic nerve axotomy was used in the experiment.The animals survived for 4,7,14,21,28 days respectively after surgery with and without MP treatment.The retinas were taken out and cut,then the number and morphological changes of RGCs with Nissl staining and the expression of Hsp27(optic density) in ganglion cell layers with immunohistochemical staining were observed respectively.The data were analysed with SAS soft ware. Results The quantity of the surviving retina ganglion cells increased in the group with high-dose intravenous methylprednisolone treatment at 7th and 14th days(P
