STUDY ON THE NEURONAL APOPTOSIS IN THE HIPPOCAMPAL CA1 REGION AFTER EXPERIMENTAL SUBARACHNOID HEMORRHAGE
- VernacularTitle:实验性蛛网膜下腔出血后海马CA1区神经元凋亡的研究
- Author:
Tao QIAN
;
Qingjun ZHANG
- Publication Type:Journal Article
- Keywords:
Subarachnoid hemorrhage;
Cerebral vasospasm;
CD95;
Hippocampus;
RT-PCR;
Laser scanning confocal microscopy;
Rat
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of neuronal apoptosis in hippocampal CA1 region and the death signaling transduction pathway initiated by CD95(Fas) molecules during cerebral vasospasm(CVS) after subarachnoid hemorrhage(SAH). Methods SAH model was developed by injecting arterial blood twice the citernamagna.The morphological changes of the neurons were studied by light microscope and laser scanning confocal microscope.RT-PCR was performed for detecting the expression changes of Fas,FasLmRNA. Results Neurons in the hippocampal CA1 region after SAH showed typical apoptotic features under light microscope and confocal microscope.The expressions of Fas,FasLmRNA in hippocampus increased in SAH group,reached the highest level 3 days after SAH and still were significantly higher 7 days after SAH compared with normal group and then decreased to normal 14 days after SAH.Conclusion The death signaling transduction pathway initiated by CD95 molecules may play important roles in hippocampus neuronal apoptosis following SAH.
