ANALYSIS OF KINDS AND ACTIVITY OF PROTEOLYTIC ENZYMES IN DIGESTIVE SYSTEM OF BUTEO BUTEO
- VernacularTitle:普通鵟(Buteo buteo)消化系统各器官蛋白水解酶种类和活性分析
- Author:
Hongxing NIU
;
Yanzhen BU
;
Yan YU
;
Yanmei WANG
;
Shengdong JI
;
Cunshuan XU
- Publication Type:Journal Article
- Keywords:
Proteolytic enzymes;
Digestive system;
G-PAGE;
Buteo buteo
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective The kinds and activities of proteolytic enzymes of tongue,esophagus,craw,muscular stomach,glandular stomach,duodenum,large intestine,pancreas,liver in digestive system of Buteo buteo were studied to provide basic information for taxonomy and evolvement study of wild birds. Methods The experiment was carried out at 4℃ by using gelatine polyacrylamide gel electrophoresis(G-PAGE) ameliorated by Xu Cunshuan. Results 1.At least 26 kinds of proteolytic enzymes were found in digestive system of Buteo buteo.2.The kinds and activities of proteolytic enzymes in the digestive system of Buteo buteo were affected by pH.The activities of proteolytic enzymes was the weakest in the acid environment and the strongest in the alkaline environment,showing that the activity of the alkaline proteolytic enzymes were stronger than that of the neutral proteolytic enzymes,and that of the acid proteolytic enzymes were weakest.3.Duodenum and pancreas had more kinds and stronger activities of proteolytic enzymes than those in tongue,esophagus and craw in any pH value condition.4.The proteolytic enzymes with 75 and 70?kD molecular weight existed in every organ of digestive system except pancreas and liver in any pH condition.Conclusion The similarity of the kinds and the activities of proteolytic enzymes exist in the organs with similar structure and similar functions. The proteolytic enzyme of 75 and 70?kD molecular weight,whose pH-dependent activity is not obvious,exists widely in digestive system of Buteo buteo.The activity of proteolytic enzyme with 198?kD molecular weight is found in every organ of digestive system under pH 8.5 condition.
