THE EXPRESSION OF SH2-B? IN THE LUNG AND VISCERAL SENSORY AFFERENT SYSTEM OF THE ASTHMATIC MICE
- VernacularTitle:哮喘小鼠肺内及内脏感觉传入系统SH2-B?的表达
- Author:
Jinping QI
- Publication Type:Journal Article
- Keywords:
SH2-B?;
Spinal ganglia;
Spinal cord;
Asthma;
Immunohistochemistry;
Western blotting Mouse
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression of SH2-B? in the lung and visceral sensory afferent system(C-7-T-5 spinal ganglia and the corresponding posterior horn of the spinal cord) of the asthmatic mice,and to explore the role of SH2-B? in asthma.Methods Thirty BALB/c mice were randomly divided into normal control group and asthmatic group on average.The alterations of SH2-B? and nerve growth factor(NGF)immunoreactivity were investigated by immunohistochemical and Western blotting method and analyzed by the Metamoph analysis system.Results Immunohistochemistry demonstrated that the mean optic density(MA)of SH2-B? positive substance in the lungs(0.806?0.023),C-7-T-5 spinal ganglia(0.766?0.014)and the corresponding posterior horn of the spinal cord(0.547?0.014)of the asthmatic mice was much higher than those(0.243?0.018,0.131?0.011,0.215?0.029) in normal control group respectively.Western blotting also demonstrated that SH2-B? and NGF expressions were more increased in the lungs and C-7-T-5 spinal cord of the asthmatic mice than those of normal control group.The mean ratio of SH2-B? MA to ?-actin MA in spinal cord and lung was 0.346?0.017 and 0.512?0.018 in normal control group,0.738?0.021 and 1.526?0.022 in asthmatic group.The mean ratio of NGF MA to ?-actin MA in spinal cord and lung was 0.357?0.021 and 0.734?0.013 in normal control group,1.445?0.015 and 1.265?0.018 in asthmatic group.A positive correlation was found between the levels of SH2-B? and NGF(r=0.651,P
