STUDY ON PROLIFERATION AND APOPTOSIS IN DEVELOPING RENAL CORPUSCLES OF MOUSE
- VernacularTitle:发育期小鼠肾小体细胞增殖与凋亡的研究
- Author:
Min GUO
;
Xu WANG
;
Huanjiu XI
- Publication Type:Journal Article
- Keywords:
Renal corpuscle;
Development;
Cell proliferation;
Apoptosis;
Immunohistochemistry;
TUNEL staining method;
Electron microscope;
Mouse
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study cell proliferation and apoptosis of renal corpuscle in normal developing mouse.Methods PCNA immunohistochemisty method,terminal deoxynucleotidyl transferase mediated dUTP-biotin nick endlabeling(TUNEL staining method)and light,electron microscopy were used to observe proliferation and apoptosis within corpuscle at different stages of development.Results Nearly all cells in Ⅰ and Ⅱ stages had nuclear PCNA immunolabeling.The number of PCNA immunolabeling cells during Ⅲ and Ⅳ stages was gradually decreased,and to less in Ⅴ stage.TUNEL positive cells could be easily observed before birth and identified in Ⅲ and Ⅳ stages.Electron microscope revealed that the mitotic activity was observed in all cells of Ⅰ and Ⅱ stages renal corpuscles.The mitotic phases of endothelial cells and podocytes could be seen in Ⅲ stage,but only endothelial mitotic activities in Ⅳ stage of renal corpuscle.Apoptotic activities of endothelial cell and Bowman's parietal cell were noted,predominantly in Ⅲ and Ⅳ stages.Conclusion Proliferation and apoptosis exist universally in the course of developing renal corpuscle,and supervise and control the regular development of renal corpuscle cooperatively.
