THE EXPRESSION OF SSEA-1 AND OCT-4 ON HUMAN EMBRYONIC GERM CELLS
- VernacularTitle:SSEA-1、Oct-4在人胚胎生殖细胞的表达
- Author:
Yongzhen CHEN
;
Qian CHEN
;
Fang LI
;
Min ZHU
;
Su ZHANG
- Publication Type:Journal Article
- Keywords:
Primordial germ cells;
Embryonic germ cells;
SSEA-1;
Oct-4;
Flow cytometry;
Human
- From:
Acta Anatomica Sinica
1954;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To culture human embryonic germ cells in vitro and identify them by observing the expression of the surface markers SSEA-1 and Oct-4. Methods The gonadal ridges and mesenteries of human embryos at 5～9 weeks post-fertilization were isolated and then cultured in vitro.Flow cytometry and immunocytochemistry were applied to detect whether the cells cultured express SSEA-1 and Oct-4.Immunohistochemistry was also carried out to observe the expression of SSEA-1 on the PGCs which locate in the gonadal ridge. Results Both the cells and the cellular colonies cultured in vitro expressed SSEA-1 and Oct-4.The immunohistology study of gonadal ridges also showed that there were many PGCs which were SSEA-1 positive in it.Conclusion The cells we obtained through tissue culturing are undifferentiated human embryonic germ cells,which originate from hPGCs.
