Electransfection with telomerase catalytic subunit(hTERT) enhances proliferative capacity of Schwann cells
10.3760/cma.j.issn.1001-2036.2010.03.017
- VernacularTitle:电穿孔法转染人端粒酶逆转录酶至许旺细胞的研究
- Author:
Cheng ZHANG
;
Biao CHENG
;
Xiaojun CHU
;
Huiren WANG
;
Zhengrong CHEN
- Publication Type:Journal Article
- Keywords:
Telomerase;
Schwann cells;
Electransfection
- From:
Chinese Journal of Microsurgery
2010;33(3):213-216,后插4
- CountryChina
- Language:Chinese
-
Abstract:
Objective To transduct with human telomerase reverse transcriptase(hTERT) to Schwann cells via electransfection technique to prolong the life span and enhance the proliferative capacity of Schwann cells.Methods The hTERT RNA was derived from esophagus cancer tissue and pcDNA3.1-hTERT vector was built.With electransfection technique, we tmnsfected vector into ADSCs.The PCR,TRAP-PCR, and PI-annexin V were tested to prove the expression of hTERT in ADSCs.Results After transduction the hTERT RNA in Schwann ceils, TRAP-PCR test, PI-annexin V dying and S100 dying was positive.Conclusion Telomerase catalytic subunit(hTERT) is transduct into Schwann cells, and it can to enhance the prolifertative capacity.
