Interactions of ovarian carcinoma cells and human peritoneal mesothelial cells involved in matrix metalloproteinases expressions of ovarian carcinoma cells
- VernacularTitle:卵巢上皮性癌细胞与腹膜间皮细胞相互作用对卵巢上皮性癌细胞基质金属蛋白酶表达的影响
- Author:
Jingjing ZHANG
;
Bo WANG
- Publication Type:Journal Article
- Keywords:
Ovarian neoplasms;
Peritoneum;
Transforming growth factor beta;
Matrix metalloproteinases
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the interactions of ovarian carcinoma cells and human peritoneal mesothelial cells (HPMC) involved in matrix metalloproteinases (MMP) expressions of ovarian carcinoma cells. Methods The conditioned medium (CM) of ovarian carcinoma cell SKOV3 was tested by enzyme-linked immunosorbent assay (ELISA) for transforming growth factor ?1 (TGF-?1). The impact of SKOV3-CM in the presence or absence of TGF-?1 neutralizing antibody on fibronectin (Fn) gene expression of HPMC was studied by RT-PCR. HPMC were pretreated with serum-free medium, SKOV3-CM, SKOV3-CM+TGF-?1 neutralizing antibody, and SKOV3-CM+IgG, then the supernatant was collected as HPMC-CM1, HPMC-CM2, HPMC-CM3 and HPMC-CM4. SKOV3 were incubated with different HPMC-CM, HPMC-CM1+antibody against Fn or HPMC-CM1+IgG. MMP-2 and MMP-9 gene mRNA expressions and protein expressions of SKOV3 were detected by RT-PCR and ELISA respectively. Results TGF-?1 in SKOV3-CM was (236?22) ng/L. Fn gene mRNA expressions of HPMC before and after stimulation by SKOV3-CM were 1.328?0.025 and 2.643?0.051, and the latter was higher than the former (P
