Identification of the Specific Proteins in Two Hydrophis with a Simple Differential Proteins Display Method
- VernacularTitle:简单差示蛋白获取法鉴别2种海蛇的应用
- Author:
Honhui ZENG
;
Yanjun YANG
;
Yajun HE
;
Qian WU
- Publication Type:Journal Article
- Keywords:
Hydrophis;
Identification;
Differential proteins display;
Specific proteins;
Western-blot
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To identify the specific proteins in two hydrophis (l#and 2#) with a simple differential proteins display method. Method By means of SDS-PAGE and Western blot, combined with analysis, the specific proteins in two hydrophis were obtained and been sequencing.Result The specific proteins of l#are 14.6kD and 8.7kD components and of 2#are 16.7kD. 10.2 kD components. The amino acid sequences of 14.6 kD and 10.2 kD components were assayed respectively. Conclusion Differential proteins can be obtained and used to identify the Chinese traditional medicinal crops by means of one-dimensional electrophoresis combined with antigen analysis .
