Determination of the Content of Astragaloside Ⅳ in Qi Zao Granules By HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定芪枣颗粒中黄芪甲苷的含量
- Author:
Huaqing LIN
;
Hong DENG
;
Shu ZHANG
- Publication Type:Journal Article
- Keywords:
Qi Zao Granules;
Astragaloside Ⅳ;
HPLC-ELSD;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of astragaloside Ⅳin Qi Zao Granules.Methods Equipped with the evaporative light scattering detector (ELSD), a RP-HPLC method was established. The chromatographic column was C18 and the column temperature was at 30 ℃.The drift tube temperature was at 40 ℃and the evaporative gas was nitrogen with pressure of 3.5 bar.The mobile phase was acetonitrile-water(30∶70) and the flow rate was 1mL/min. Results This method showed a good linear relationship in the range of 2.51～50.2 ?g astragaloside Ⅳ. The average recovery was 98.13 %with RSD being 1.28 %. Conclusion The method is sensitive and accurate and can be used for the quality control of Qi Zao Granules.
