Effects of exercise on the expression of glycogen synthase kinase-3 in the skeletal muscles of insulin resistant rats
- VernacularTitle:运动对胰岛素抵抗大鼠骨骼肌中糖原合成酶激酶-3表达的影响
- Author:
Dan CHEN
;
Huimin BI
;
Xin BI
- Publication Type:Journal Article
- Keywords:
Exercise;
GSK-3;
Insulin resistance;
Animal model
- From:
Chinese Journal of Physical Medicine and Rehabilitation
2003;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of exercise training on the expression of glycogen synthase ki-nase-3 (GSK-3) in the skeletal muscles of rats with insulin resistance induced by a high-fat and high sucrose diet. Methods Thirty Wistar rats were randomly divided into three groups: a control group, a model group and an exercise group. Rats in the model and exercise groups were fed with a high-fat and sucrose diet (20% sucrose, 10% lard, 2.5% cholesterol, 1% cholic acid) for 4 weeks, and then the rats in the exercise group were trained by swimming 5 d every week for 6 weeks. After treatment, the concentration of GSK-3 protein in their muscles was detected by Western blot analysis, while their weights, serum triglyceride and total cholesterol, and their concentrations of fasting plasma glucose and fasting plasma insulin were measured periodically and their insulin sensitivity index was calculated. Results Compared with the controls, the expression of GSK-3 in the skeletal muscles of the rats in the model group increased by 70. 20% (P
