Chromatographic fingerprints of Andrographis paniculata (Burm.f.) Nees.collected from different places
- VernacularTitle:不同产地穿心莲药材色谱指纹图谱的比较研究
- Author:
Haijuan DONG
;
Zunjian ZHANG
- Publication Type:Journal Article
- Keywords:
Andrographis paniculata(Burm.f.) Nees;
fingerprints;
HPLC;
hierarchical clustering;
nonlinear mapping;
similarity
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the HPLC fingerprints and establish a sensitive and specific method for the quality control of Andrographis paniculata(Burm.f.) Nees. METHODS: All 23 samples of Andrographis paniculata(Burm.f.) Nees collected from 10 different places were determined by RP-HPLC.Shimadzu-ODS column was used with maxtures of 0.1% formic acid-acetonitrile and 0.2% formic acid-water as mobile phase in gradient mode.The flow rate was set at 1.0 mL/min.The column temperature was set at 25℃ and detecting wavelength at 254 nm.Hierarchical clustering,nonlinear mapping and similarity criteria were applied to evaluate the fingerprints. RESULTS: The simple and specific method with good repeatability was established.There were different contents of each component contained in habitat samples produced in individual area. CONCLUSION: The proceeding is suitable to differentiate Andrographis paniculata(Burm.f.) Nees from different places conveniently and can be used for the quality control of this herb.
