Determination of ginsenoside Re、ginsenoside Rg_1 and schisandrin in Huoliyuan Tablets by HPLC
- VernacularTitle:HPLC测定活力源片中人参皂苷Re、Rg_1及五味子醇甲的含量
- Author:
Xiangyang LI
;
Wanqian TU
- Publication Type:Journal Article
- Keywords:
HPLC;
Huoliyuan Tablets;
ginsenoside Re;
ginsenoside Rg_1;
schisandrin;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a HPLC method for determining of ginsenoside Re,ginsenoside Rg_1 and schisandrin in Huoliyuan Tablets(total ginsenoside,Radix Astragali,Fructus Schisandrae Chinensis,Radix Ophiopogonis,etc.). METHODS: A Hypersil C_(18) column was used.The mobile phase for ginsenoside Re and Rg_1 was acetonitrile-water mixed with 0.05 mol/L NaH_2PO_4(pH2.7,20∶80),and determination wavelength was 203 nm.The mobile phase for schisandrin was acetonitrile-0.1%H_3PO_4(48∶52),and determination wavelength was 250 nm. RESULTS: The linear ranges of ginsenoside Re,Rg_1 and schisandrin were 1.84-14.72 ?g(r=0.999 4),1.49-11.92 ?g(r=0.999 0) and 0.094-0.47 ?g(r=0.999 9),respectively.The average recoveries of ginsenoside Re,Rg_1 and schisandrin were 99.86%(RSD=1.69%),98.17%(RSD=2.03%) and 101.54%(RSD=(2.62%),) respectively. CONCLUSION: The method is simple,accurate,and can be used for the quality control of Huoliyuan Tablets.
