Effect of chemotherapy drug on protein expression of pancreas cancer cell lines SW-1990
- VernacularTitle:化疗药物对胰腺癌细胞株SW-1990蛋白质谱表达的影响
- Author:
Zhaoshen LI
;
Yanfang GONG
- Publication Type:Journal Article
- Keywords:
Proteomics;
Pancreatic cancer;
Gemcitabine;
5-FU
- From:
Chinese Journal of Digestion
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objectives To verify if chemotherapy drug gemcitabine could induce a change of protein expression in pancreatic cancer cell line SW-1990, and to provide evidence for clinical therapy. Methods We use two-dimensional gel eleclrophoresis(2-DE), mass speclrornetry and bio-information methods to compare the change of protein expression before and after gemcitabine and 5-FU treatment of cell line SW-1990, and identify the discrepant protein expression. Results The concentration of gemcitabine, that can suppress 50 percent of the pancreatic cancer cells was 1-100 ng/ml, while same suppression for 5-FU was 250-2500 ng/ml. Six discrepant protein expressions, one in 5-FU group and 5 in gemcitabine group were found. Conclusions Gemcitabine has better therapeutic effects on the growth of pancreatic cancer cell line SW-1990 than that of 5-FU. Gemcilabine was involved in sugar and fat metabolism, and it could induce some changes of protein expression, for example ?-actin and MGC: 19713, which is of somewhat importance for clinical investigation of pancreatic cancer therapy.
