Determination of Notoginsenoside R_1 and Ginsenoside Rg_1, Rb_1 in Naodesheng Capsules by HPLC Gradient Elution Method
- VernacularTitle:高效液相色谱法测定脑得生胶囊中三七皂苷R_1、人参皂苷Rg_1及人参皂苷Rb_1的含量
- Author:
Yonghui SUN
;
Xiaoli WANG
;
Jianming WANG
- Publication Type:Journal Article
- Keywords:
Naodesheng Capsules;
Notoginsenoside R1;
Ginsenoside Rb1;
Ginsenoside Rg1;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of HPLC gradient elution method determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules. Methods Kromasil C18 column was used with a mobile phase of acetonitrile-water gradient elution, column temperature at 25 ℃. Results Notoginsenoside R1, Ginsenoside Rb1 and Ginsenoside Rg1 showed good linear relationship at the range of 0.495～1.782 ?g (r =0.9999), 2.65～9.54 ?g (r =0.9995), 1.85～6.66 ?g (r =0.9999), respectively. The average recovery:Notoginsenoside R1 was 100.60% (RSD=1.99%), Ginsenoside Rb1 was 100.86% (RSD=2.19%) and Ginsenoside Rg1 was 98.83% (RSD=1.95%), respectively. Conclusion The methods is simple, accurate, highly sensitive and reproducible. It can be used for the quantitative determination of Notoginsenoside R1, Ginsenoside Rg1 and Rb1 in Naodesheng Capsules.
