Effect of procyanidins on gene expression of par-4 and bcl-2 in PC12 cells induced by A?_(25-35)
- VernacularTitle:原花青素对?-淀粉样肽25-35诱导PC12细胞par-4和bcl-2基因表达的影响
- Author:
Hanfang MEI
;
Zhaoyang XIE
;
Hong YANG
;
Qifeng ZHU
- Publication Type:Journal Article
- Keywords:
procyanidins (PC);
?-amyloid peptide_(25-35) (A?_(25-35));
PC12 cells;
apoptosis;
par-4;
bcl-2
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of procyanidins (PC) on mRNA and protein expression of par-4 and bcl-2 genes in PC12 cells induced by A?_ 25-35 . Methods Cell survival rate was evaluated by MTT assay and apoptosis was analyzed by Hoechst 33258-PI fluorescence staining. The expressions of mRNA and protein for par-4 and bcl-2 were tested by RT-PCR and Western blotting. Results Pretreatment with different concentrations of PC (5、10、20, and 30 mg/L) for 1 h increased the survival rate of PC12 cell in a dose-dependent manner. PC prevented the PC12 cells nuclei from shrinkage, condensation, and cleavage induced by A?_ 25-35 . PC decreased the expression of par-4 mRNA and protein, and increased the expression of bcl-2 mRNA and protein. Conclusion PC can protect PC12 cells from apoptosis induced by A?_ 25-35 in a dose dependent manner. The mechanism of protection is likely related to decreasing the par-4 gene expression and increasing the bcl-2 gene expression.
