HPLC Fingerprint of Pollen Typhae
- VernacularTitle:蒲黄的HPLC指纹图谱研究
- Author:
Hongfei MA
;
Bin LIU
;
Junting ZHOU
- Publication Type:Journal Article
- Keywords:
Pollen Typhae;
HPLC;
fingerprint;
similarity
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC Fingerprint of Pollen Typhae and give a new method for quality control of Pollen Typhae. Methods RP-HPLC was used on a YWG-C_ 18 column (250 mm?4.6 mm, 5 ?m) with the gradient elution solvent system composed of acetonitrile-0.05% H_3PO_4 water solution, the detection wavelength was 254 nm, sample injection was 5 ?L. Results HPLC Fingerprint of Pollen Typhae was established. Taking typhaneoside as the reference peak, 12 common peaks were selected as the fingerprint peaks of Pollen Typhae, the similarity between the fingerprint of eight batches of Pollen Typhae samples and reference fingerprint was determined. Conclusion The established HPLC fingerprint has desirable precision, reproducibility, and stability, and can be applied to the quality control of Pollen Typhae.
