Protective effects of Jidesheng Sheyao tablet on endotoxin-induced acute lung injury in rats
- VernacularTitle:季德胜蛇药片对大鼠急性肺损伤的保护作用
- Author:
Dongying TAO
;
Pingsheng CHEN
;
Dongfeng LIU
- Publication Type:Journal Article
- Keywords:
Jidesheng Sheyao tablet;
Matrix metalloproteinase;
Acute lung injury;
Lipopolysaccharide
- From:
Chinese Journal of Emergency Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the protective role of Jidesheng Sheyao tablet in lipopolysaccharide (LPS)-induced acute lung injury(ALI) in rats. Methods Fifty male SD rats were randomly divided into 6 groups:normal control group,dexamethasone positive group,LPS model group, Sheyao groups of low, middle and high dosages successively.All rats, except normal control group, were administrated with LPS by intravenous injection to induce acute lung injury. The rats in positive group and three different dosage groups were treated by dexamethasone(3 mg?kg -1, iv), low dosage(0.8 g?kg -1), middle dosage(1.6 g?kg -1) and high dosage(3.2 g?kg -1) of Jidesheng Sheyao tablet(oral) respectively before LPS-induced ALI. Each rat in normal control group received injection of 1ml normal saline. The animals were killed after injection of LPS for 2 hours, and then the lung index was calculated, the histopathology of the lung injury was observed by light microscope, the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) proteins in the lung tissues and their activity in the bronchoalveolar lavage fluid (BALF) and the lung homogenates were detected by immunohistochemistry and zymography separately. Results Compared with model group, the lung indexes were significantly decreased in the drug groups(P
