Inhibitory effects of c-raf-1 antisense oligodeoxynucleotides transfactionon proliferation of the human ovarian carcinoma SKOV_3 cell lines
- VernacularTitle:c-af-1反义寡核苷酸转染对人卵巢癌SKOV_3细胞增殖的抑制作用
- Author:
Yongzhong WU
- Publication Type:Journal Article
- Keywords:
SKOV3 cell lines;
Antisense oligodeoxynucleotides;
Ovarian carcinoma;
c-raf-1
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effects and the mechanisms of c-raf-1 genes antisense oligodeoxynucleotides(ASODN) transfection in inhibiting the human ovarian carcinoma SKOV3 cell lines.Methods: There were 3 groups in our study: normal control group,c-raf-1 sense oligodeoxynucleotides(SODN) experimental group,and c-raf-1 antisense experimental group.at the different time points after liposome-mediated transfection,the cell proliferation,apoptosis,protein expressing level were observed by MTT assay,flow cytometry,fluorescent microscope and cloning test.Results: In the ASODN experimental group and SODN group,the OD-value were 0.272 and 1.307 respectively(P
