Experimental study on inhibitory effects of PEDF on rat cornealneovascularization after penetrating keratoplasty
- VernacularTitle:PEDF抑制大鼠角膜移植术后新生血管作用的实验研究
- Author:
Qiaoling WANG
- Publication Type:Journal Article
- Keywords:
Pigment epithelium derived factor;
Penetrating keratoplasty;
Corneal neovascularization
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objectve: To evaluate the effects of pigment epithelium derived factor(PEDF) eyedrops in inhibiting corneal neovascularization(CNV) by topical installition on the rat model undergoing penetrating keratoplasty and to investigate its possible mechanism and its clinical significance.Methods: Penetrating karatoplasty were proformed orthotopically from Wistar rats to SD rat' recipients.72 SD rats were randomly assigned to two groups-experimental group and control group,after transplantation.Experimental group was treated with PEDF eyedrops and control group was treated with escipient eyedrops.CNV was observed by the slitlamp biomicroscope examination.The area of CNV was caculated quantitatively.6 animals in both groups were killed respectively at the 1st week,2nd week,3rd week,1st month,2nd month and 3rd month postoperatively.PEDF expression was examined by immunohistochemistry and corneal untrastructural morphology was observed by TEM.Results: The area of CNV was decreased at 1 week,2 weeks,3 weeks,1 month and 2 months postaperatively(P
