A study on apoptosis in isolated pancreatic islet cells induced cytokines and its mecanism
- VernacularTitle:细胞因子诱导离体胰岛?细胞凋亡及其机理研究
- Author:
Huarong YU
- Publication Type:Journal Article
- Keywords:
Interleukin-1?;
Apoptosis;
Nitric oxide;
Gene expression;
Islet cell
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the apoptosis of isolated pancreatic islet cells and insulin secretion induced by combination of IL-1?,TNF and IFN-?,and to investigate the mechanism of the change.Methods: Isolated pancreatic islet cells from Wistar rat were incubated in monolayer in vitro.NO production,NOS activity,insulin secretion,the protein expression of bcl-xl and bax,apoptotic rate of islet cells and DNA fragmentation in pancreatic islet cells incubated with combination of IL-1?,TNF and IFN-? were measured.Results: Combination of IL-1?,TNF and IFN-? induced a significant increase in apoptotic percentage of pancreatic islet cells,NO production and NOS activity,as well as DNA ladder appearance,while a decrease in insulin content.Up-regulation of the protein expression of bax and down-regulation of the protein expression of bcl-xl were also induced(P
