Genotyping of Stenotrophomonas maltophilia by using repeat sequence PCR
- VernacularTitle:应用重复序列PCR技术对嗜麦芽窄食单胞菌进行基因分型
- Author:
Yun XIA
- Publication Type:Journal Article
- Keywords:
Stenotrophomonas maltophilia;
Polymerase chain reaction;
Genotyping.
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a rapid,simple and accurate genotyping method for the epidermical investigation of nosocomial infection caused by Stenotrophomonas maltophilia,and to explore the relationship between the genotyping and the drug-resistant profiles.Methods: Fourteen strains of Stenotrophomonas maltophilia isolated from clinic were genotyped by the techenic of Rep-PCR with the primer of enterobacterial repetitive intergenic consensus(ERIC),and the results of Rep-PCR were analyzed and compared with the patterns of antibiotic susceptibility.Results: All 14 strains involved in this study exhibited nine antibiotic susceptibility patterns.Of which 13 strains exhibited 5 different genotypes.There were no defined relationship between the results of the genotyping and the drug-resistant profiles.Conclusion: The Rep-PCR techenic used in this study is simple and repreducible for genotyping of Stenotrophomonas maltophilia.It is valuable in the molecular epidemiological investigation of nosocomial infection.
