Determination of triptolide in the sustained-release capsule ofTripterygium Wilfordii Hook.f.with HPLC
- VernacularTitle:HPLC法测定雷公藤缓释胶囊中雷公藤内酯醇的含量
- Author:
Lurong YU
- Publication Type:Journal Article
- Keywords:
Tripterygium Wilfodii;
Sustained-release capsule;
Triptolide;
Content determination
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish a method for content determination of triptolide in the sustained-release capsule of Tripterygium Wilfordii by high performance liquid chromatography.Methods: High performance liquid chromatography was performed on C18 column.The chromatographic conditions were as follows: Acetonitrile-water(40:60) as mobile phase;flow rate being 1.0 ml/min and the detecting wavelength at 220nm.Results:Triptolide had good linear relation within the range from 0.047 to 0.350?g/ml(r=0.9995).The average recovery was 99.88%(RSD=0.58%,n=5).Conclusion: This method is convenient,accurate,reproducible,which can be used for content determinaton of triptolide.
