Determination of selenium in serum with Luminol-H_2O_2-KMnO4 chemiluminescence system
- VernacularTitle:静态化学发光淬灭分析法测定血清硒
- Author:
Gang YI
- Publication Type:Journal Article
- Keywords:
Luminol;
Chemiluminescence;
Selenium
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To study the quenchable effect of Selenium to Luminol-H2O2-KMnO4 Chemiluminescence system and to establish a method for the determination of Selenium in serum.Methods: Chemiluminescence system was used to determine the Chemiluminogenic strength of Luminol-H2O2-KMnO4 and the detection of Selenium in serum was realized.Results:A detection limit with 6.5?10-4?g/ml and a 1.5?10-3～6.5?10-1?g/ml linear range were obtained.The average recovery fraction was 94.21% and the relative standard deviation was 2.97%.Conclusion: A satisfactory result for the determination of Selenium in serum by this method is obtained.
