Expression of PDGF and growth of VSMC after mechanical injury and exposure to autologous serum
- VernacularTitle:机械损伤加自体血清对VSMC生长及其自分泌PDGF的影响
- Author:
Huanzhang NIU
;
Qin LU
;
Yanli AN
;
Meng PAN
;
Gaojun TENG
- Publication Type:Journal Article
- Keywords:
VSMCs;
Restenosis;
PDGFs
- From:
Journal of Interventional Radiology
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the growth and expression of PDGF of VSMCs in response to stimulation of autologous serum and mechanical injury. Methods An vitro model simulating the condition as possible as that after PTA.PDGF of every medium sample from every group was detected by ELISA,and the values of MTT of every cellular sample was measured by MTT to show the growth and proliferation of every group. Results After stimulation by autologous serum and mechanical injury,SMCs of the experimental group showed the value of MTT increasing,but SMCs in control group reached on 3rd day.At the same time,the expression of PDGF also increased gradually,obtaining peak gradually up to peak on day 4/5 nearly 2.0-fold as much as that of SMCs in the control group. Conclusions After on the 5th day,stimulation with autologous serum and mechanical injury,VSMCs of rabbit showed the stronger ability of growth/proliferation,and autocrine of PDGF also increased gradually,reaching peak on 4-5 d,probobly simulating to those in vivo.
