Establishment and application of fingerprints of Shuanghuanglian Powder-Injection by HPLC
- VernacularTitle:双黄连粉针剂高效液相指纹图谱的建立及应用
- Author:
Baiping MA
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian Powder-Injection;
high performance liquid chromatography;
fingerprints
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the fingerprints of Shuanghuanglian Pwder-Injection(Flos Lonicerae Japonicae,Radix Scutellariae,Fructus Forsythiae) by high performance liquid chromatography(HPLC) so as to control quality of the injection. METHODS: The experimental conditions of the HPLC method were as follows: YMC-Pack ODSA C_(18) column(4.6 mm?250 mm,5 ?m,mobile phase consisting of methanol and 0.012 5 N ammonium acetate/acetic acid buffer eluted with a linear gradient,flow rate at 1.0 mL/min,the detectors of phtodiode array detector(PDA) and evaporative light scattering detector(ELSD) connected in series. RESULTS: The method provided two kinds of fingerprints with excellent spearation,which were HPLC-PDA(254 nm) fingerprint and HPLC-ELSD fingerprint.They had a good correlation and complementation each other.Baicalin was used as a standard substance.Thirteen common peaks were the picture of fingerprints features.The results showed the coincidence of the quality in the total ten batches of the injections. CONCLUSION: The method is stable,accurate and creditable,meanwhile it provides much useful information for the quality control of Shuanghuanglian Powder-Injection.
