Expression of X-linked inhibitor of apoptosis in pancreatic carcinoma tissues and its relationship to chemoresistance
- VernacularTitle:X染色体连锁的凋亡抑制蛋白在胰腺癌组织及细胞中的表达及与化疗耐药的关系
- Author:
Zhiyuan JIAN
;
Yixiong LI
;
Xiaogang LI
- Publication Type:Journal Article
- Keywords:
Pancreatic carcinoma;
X-linked inhibitor of apoptosis;
Chemoresistance
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of X-linked inhibitor of apoptosis (XIAP) in pancreatic carcinoma tissues and SW1990 cell line, and to investigate its relationship to the chemoresistance. Methods The expression of XIAP in 23 pancreatic carcinoma tissues was detected by immunohistochemistry and the relationship of XIAP expression with the clinical stage and pathological grade was analyzed. 5-FU was used to induce the chemoresistant capability of SW1990 pancreatic cell line. The XIAP expression in SW1990 was examined by RT-PCR and Western blot. The XIAP level in the cells and its chemoresistance to 5-FU were analyzed by linear regression. Results The XIAP expression was detected in 89. 6% (20/23) of pancreatic carcinoma tissues and 33. 3% (4/12) of normal pancreatic tissues,respectively. The positive rate and expression level of XIAP were higher in pancreatic carcinoma than normal pancreatic tissues. In addition, 5-FU could induce the chemoresistance (P
