Polymorphic gene regulation of UDP-glucuronosyltransferase 1A in human colorectal epithelium and liver
- VernacularTitle:葡萄糖醛酸转移酶1A基因的组织差异性表达及多态调节
- Author:
Min WANG
;
Yanqing LI
;
Jian CHEN
- Publication Type:Journal Article
- Keywords:
Colorectal carcinoma;
UDP-glucuronosyltransferase;
Gene;
Polymorphism
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze tissue-specific expression and polymorphic regulation of the UDP - glucuronosyltransferase (UGT) 1A gene in human tissue. Methods Forty colorectal cancer tissue. 20 normal colorectal tissue and 10 normal liver tissue were studied. UGT 1A transcriptions were investigated by exon-1 specific RT-PCR. UGT 1A proteins were studied using Western blotting analysis. Meanwhile catalytic activity of microsomes was evaluated using N-hydroxy-PhiP. Results (1) UGT 1A mRNA expressions were found to be significantly down-regulated in colorectal cancer tissue as compared with the surrounding normal tissues(P
