The influence of T regulatory cells on experimental colitis of rate after treatment by probiotics
- VernacularTitle:益生菌治疗的实验性结肠炎中调节性T细胞的作用
- Author:
Ning CHEN
;
Peng YOU
;
Annan LIU
- Publication Type:Journal Article
- Keywords:
Probiotics;
Trinitrobenzenesulphonic acid;
Colitis;
T lymphocyte, Regulation
- From:
Chinese Journal of Digestion
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effects of probiotics on experimental colitis of rats and to explore systemic and local changes of T regulatory cells(Tr) after the treatment. Methods The models of experimental colitis were established by enema with 2,4,6-trinitrobenzenesulphonic acid (TNBS). Live probiotics of combined bifidobacterium,lactobacillus and enterococcus (Bifico) were used. Four groups of rats were set up as negative group, TNBS alone group, probiotics group (high dosage and low dosage, with dose of 150 and 300 mg?kg-1?d-1, respectively), and combination group (probiotics plus prednisolone or sulfasalazine). Histologic scoring was used to evaluate the effect of medications; the proportions of CD4+CD25+ and CD8+CD28- Tr in peripheral blood, spleen mononuclear cells and intraepithelial lymphocytes of colon were detected by flow cytometry. Results Histopathology showed that high dosage of probiotics was effective in TNBS-induced colitis rats (2. 2?0.8 vs 3.5?0.7,P
