The levels of serum tumor necrosis factor-alpha and soluble tumor necrosis factor receptor of patients with severe lupus nephritis and the effect of double pulse therapy with methylprednisolone combined cytoxan on them
- VernacularTitle:重症狼疮肾炎患者血清TNF-?与sTNF-R水平及甲泼尼龙与环磷酰胺双冲击治疗对其的影响
- Author:
Chengyun XIA
;
Jingguo ZHOU
;
Guoyuan ZHANG
- Publication Type:Journal Article
- Keywords:
Lupus nephritis;
Tumor necrosis factor-alpha;
Soluble tumor necrosis factor receptor;
Methylprednisolone;
Cytoxan
- From:
Chinese Journal of Rheumatology
2001;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the levels of serum tumor necrosis factor-alpha (TNF-?琢) and soluble tumor necrosis factor receptor (sTNF-R) and sTNF-R/TNF-?琢 ratios in patients with severe lupus nephritis (SLN) and the impact of double pulse therapy with methylprednisolone (MP) combined cytoxan (CTX). Methods Serum TNF-?琢, soluble tumor necrosis factor receptor type Ⅰ(sTNF-RⅠ) and soluble tumor necrosis factor receptor type Ⅱ (sTNF-RⅡ) levels were determined in 38 cases of SLN patients and 35 health controls by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) before and after the double pulse therapy with MP and CTX. Anti-dsDNA antibody was detected by ELISA. Antinuclear antibody (ANA) was detected by indirect immunofluorescence experiments. Complement C3 and complement C4 were detected by rate nephelometry. Results The serum levels of TNF-?琢, sTNF-RⅠ, sTNF-RⅡand ratios of TNF-?琢/sTNF-RⅠ, TNF-a/sTNF-RⅡ were significantly higher in SLN patients than those in normal control group (P
