Fingerprints of soybean total isoflavone by HPLC
- VernacularTitle:大豆总异黄酮HPLC指纹图谱研究
- Author:
Rong SHI
;
Shaoyun WANG
;
Weilin JIANG
;
Lei NIE
;
Zhun HOU
- Publication Type:Journal Article
- Keywords:
Glycine max L.Merr.(soybean);
isoflavone;
fingerprints;
HPLC
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the HPLC fingerprints of isoflavones from different medicinal plants of G.max and establish a sensitive and specific method for controlling the quality of the soybean isoflavone.Methods The gradient elution mode was applied in chromatographic separation.A C_(18) column(250 mm?4.6 mm,5 ?m) was used with the mobile phase: ice acetic acid water solution(pH value 3.2)-methanol,flow rate: 0.6 mL/min,detecting wavelength: 261 nm,and the column temperature: room temperature.All 25 samples collected from different species were determined.The clustering analysis and the software of similarity analysis were applied for datum analysis.Results This method had a good repeatability and reproducibility.The ratio of peaks′ area from distinct samples were different.Conclusion The method can show the difference of chemical compositions among species completely and can be used as a quality control method for soybean.
