Influence of biotic and abiotic elicitors on production of tanshinones in Salvia miltiorrhiza hairy root culture
- VernacularTitle:生物和非生物诱导子对丹参毛状根培养生产丹参酮的影响
- Author:
Qiong YAN
- Publication Type:Journal Article
- Keywords:
the hairy root of Salvia miltiorrhiza Bunge;
tanshinones;
biotic elictor;
abiotic elicitor
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare influence of several biotic elicitors(yeast extract,(oligogalactu-)ronides,and fungal elicitor) and abiotic elicitors(Ag~+,Co~(2+),and ?-amino isobutyric acid) on tanshinone production in the hairy root culture of Salvia miltiorrhiza.Methods The contents of three tanshinones were(analyzed) by high performance liquid chromatography.Results Biotic elicitors increased effectively the yield of tanshinones and there were difference among different elicitors' effects.The highest content of tanshinones was achieved by fungal elicitor at 100 ?g/mL,which was(0.42 mg/g),4.7-fold of the control.The elicitation effects of abiotic elicitors were relatively lower than those of biotic elicitors,with Co~(2+) at 50 ?mol/L giving the highest content of tanshinone(1.75 mg/g,4.1-fold of the control) among abiotic elicitors.Biotic and abiotic elicitors showed the selectivity of elicitation.All biotic elicitors notably increased the crypotanshinone content and all abiotic elicitors selectively enhanced the tanshinone I content.And in most cases,the elicitors added did not cause any obviously inhibitory effect on the hairy root growth. Conclusion Adding different biotic and abiotic elicitors may selectively promote tanshinone accumulation and not affect the hairy root growth in the hairy root cultures of S.miltiorrhiza.
