Apoptosis of human leukemia K562 cells induced by snake venom of Agkistrodon Halys Pallas in Zhejiang Province
- VernacularTitle:浙江蝮蛇蛇毒诱导人白血病K562细胞凋亡
- Author:
Qinghua DONG
;
Shu ZHENG
;
Qinghua LU
;
Liming HE
- Publication Type:Journal Article
- Keywords:
Snake venom of Agkistrodon Halys Pallas;
Ieukemia;
Apoptosis;
Bcl-2
- From:
Chinese Journal of Emergency Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the apoptosis of human leukemia K562 cells induced by snake venom of Agkistrodon Halys Pallas in Zhejiang Province.Methods IC_ 50 value and cytotoxity of K562 cell were detected by MTT method.Apoptotic cells were dyed by Hoechest 33258.Sub-G1 peak and cell cycle were detected by FCM.Protein expression of Bcl-2 gene was detected by FCM and western-blot method.Results The snake venom of Agkistrodon Halys Pallas in Zhejiang Province inhibited the growth of K562 cells,which appeared dose-dependent.The snake venom induced apoptosis of K562 cells.Meanwhile,protein expression of Bcl-2 was down-regulated.Conclusion Snake venom of Agkistrodon Halys Pallas in Zhejiang could induce apoptosis of human leukemia K562 cells.The mechanism may be related to down-regulation of Bcl-2 gene expression.
