EFFECT OF NERVE REGENERATION FACTOR ON THE NEURITE GROWTH OF RAT DORSAL ROOT GANGLIONS
- VernacularTitle:神经再生素促大鼠背根神经节生长作用的研究
- Author:
Qi ZHANG
;
Xin TANG
;
Fei DING
- Publication Type:Journal Article
- Keywords:
Nerve regeneration factor;
Dorsal root ganglion;
Neurofilament;
real-time flourescence quantitative RT-PCR;
Immunofluorescence;
Cytochemistry;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of Nerve Regeneration Factor on the neurite growth and Neurofilament-H expression of rat dorsal root ganglions in vitro. Methods DRG was cultured and treated with NRF,observation made by fluorescent immunocytochemistry and the mRNA and protein level of NF-H of DRG neurons was detected by real-time fluorescence quantitative RT-PCR and Western blot. Results NRF can promote the neurite growth of DRGs in vitro.The mRNA and protein levels of NF-H of DRG neurons can be up-regulated by NRF in a dose-dependent manner,with the best concentration of 2.0?(mg/L.) Conclusion\ NRF can promote the neurite growth of DRGs in vitro.NRF exerts a neurotrophic action on developmental sensory neurons.
