THE EFFECT OF ?-ELEMECE ON REVERSAL MULTIDRUG RESISTANCE AND THE EXPRESSION OF P-GLYCOPROTEIN IN K562/ADM
- VernacularTitle:?-榄香烯对K562/阿霉素细胞多药耐药性的逆转及其对P-糖蛋白表达的影响
- Author:
Lihong HAO
;
Jinyao ZHAO
;
Chuanzhou GAO
;
Yanfang DING
;
Peiman YANG
- Publication Type:Journal Article
- Keywords:
Resistance-reversing;
P-glycoprotein;
?-elemene
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of ?-elemece on the reverse of drug resistance and the expression of P-glycoprotein(P-gp) in MDR K562/ADM cells. Methods MTT method was used to detect the sensitivity of cell and its reverse of drug resistance.Immunohistochemistry and immunoelectron microscopy were for observing the expression of P-gp.It's quantitative analysis was determined by flow cytometry.All experimental data were dealt with by SPSS(10.0 production pacility) soft ware. Results 1.Non-cytotoxic dose of ?-elemece(4.0?mg/L) could obviously decrease the IC-(50) value of K562/ADM cells to ADM.The reversing fold was 2.18;2.The level of P-gp expression was higher in K562/ADM cells than that in its parent K562 cells.Our studies also showed that the distribution of P-gp was on membrane of K562/ADM cells;3.Non-cytotoxic dose of ?-elemece(4.0?mg/L) could remarkably decrease the P-gp expression in MDR K562/ADM cells.Conclusion\ ?-elemece could evidently reverse drug resistance of K562/ADM to ADM.Decreasing the P-gp expression in drug resistance cell is one of the main mechanism of reversion.
