THE EXPRESSION OF P75 IN HEPATIC STELLATE CELLS OF FIBROTIC HUMAN AND RAT LIVER
- VernacularTitle:神经生长因子低亲合力受体在肝纤维化患者和大鼠肝星状细胞的表达
- Author:
Xin CHEN
;
Huiqing JIANG
- Publication Type:Journal Article
- Keywords:
Hepatic fibrosis;
HSCs;
P75 expression;
Immunochemically assay;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the expression site and the role of low-affinity nerve growth factor receptor(P75) in hepatic stellate cells(HSCs) of fibrotic human and rat liver.Methods Left lobe of liver was removed from CCl-4-induced rats fibrosis model and human fibrotic tissue sample was taken from liver puncture.The tissue specimen was routinely paraffin embedded and expression of P75 was detected by histochemical staining using polyclonal antibody against P75.Additionally,HSCs was cultivated and P75 was immunochemically assayed for P75 binding activity. Results P75 was seen abundantly on cell membrane of the cultured HSCs.In the fibrotic tissue of rat liver as well as human fibrotic liver P75 was also positively expressed.Similarly,P75 was found mainly a hepatocye surface in histochemical staining.P75 was not seen by histochemical staining in the normal tissue of human and rat liver.Conclusion P75 used as a new target for the treatment of hepatic fibrosis.
