COMPOUND STAINING METHOD SHOWING OSSIFIED SKELETAL STRUCTURES AND CARTILAGE IN FETAL, NEONATAL AND ADULT RAT
- VernacularTitle:整体显示大鼠骨骼和软骨的复合染色法
- Author:
Ying ZHAO
- Publication Type:Journal Article
- Keywords:
Ossified bone;
Cartilage;
Alizarin red S;
Alcian blue;
Compound staining;
Rat
- From:
Acta Anatomica Sinica
1953;0(01):-
- CountryChina
- Language:Chinese
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Abstract:
Objective To compare compound staining of Alizarin red S and Alcian blue showing ossified skeletal structures and cartilage in fetal,neonatal and adult rat. Methods All specimens were skinned and fat tissue removed completely and eviscerated,then fixed in 95% ethanol;Acetone was used remove fats; 0.1% Alizarin red S and 0.15%-0.3% Alcian blue were used in ossified bone and cartilage;Differentiation and clearing in 0.5%-2% aqueous KOH. Results Ossified bones showed rose red;cartilage showed blue;the others were transparent.Conclusion The best results of Alizarin red S and Alician blue compound staining could be obtained in all rat skinned,eviscerated,and removed the adipose tissues.
