Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 mRNA in endometriosis
- VernacularTitle:基质金属蛋白酶9及其组织抑制剂1在子宫内膜异位症组织中的表达
- Author:
Yan LI
;
Jinghe LANG
- Publication Type:Journal Article
- Keywords:
Endometriosis;
Gelatinase B;
Tissu-inhibitor of metalloproteinase-1
- From:
Chinese Journal of Obstetrics and Gynecology
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate mRNA expression of matrix metalloproteinase (MMP-9) and tissue inhibitor of metalloproteinase (TIMP-1) in ectopic endometriosis tissue and uterine endometrium from women with and without endometriosis. Methods Thirty-eight women with endometriosis (Revised American Fertility Society classification, RAFSⅠ-Ⅳ) were selected as study group. Thirty-eight specimens of ovarian endometrioma(ovarian chocolate cysts, OCC), 16 red peritoneal endometriotic lesions(RPL), and 35 matched eutopic endometrium(Eu) were collected from them simultaneously at the time of surgery. Twenty specimens of endometrium from reproductive women undergoing laparoscopic surgery without endometriosis were obtained as control group. The mRNA expressions of MMP-9 and TIMP-1 were detected by reverse transcription polymerase chain reaction (RT-PCR). Results Expression of TIMP-1 mRNA was detected in all samples. The level from endometriosis patients and control group was similar(2.31?1.21, 2.40?0.89). However, ectopic endometrium expressed significantly fewer TIMP-1 mRNA(OCC 1.67?0.79, RPL 1.45?0.68)compared with eutopic endometrium from both endometriosis and endometriosis-free patients(P
