Separation and Determination of Puerarin in Laige Granules by HPLC
- VernacularTitle:HPLC法测定莱葛颗粒中葛根素的含量
- Author:
Shiguo HOU
;
Shuyan WANG
;
Hongxia LI
;
Jinling CHAI
;
Xuexin GU
- Publication Type:Journal Article
- Keywords:
Laige Granules;
Puerarin;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a RP- HPLC method for quantitative determination of puerarin in Laige Granules. Methods The separation was performed on Econosphere C18 column (4.6 mm? 250 mm,5 ? m) with mobile phase of methanol and 1 % acetic acid solution (25 ∶ 75) at a flow rate of 0.8 mL/min. The UV detection wavelength was 250 nm and the column temperature was 40 ℃ . Results The linear range of puerarin was 0.2～ 1.0 ? g, r = 0.999 7. The mean recovery was 100.56 % (RSD=1.21 % , n = 5 ). Conclusion This method is simple, accurate, sensitive and with good reproducibility .
